filter matrix 和 raw matrix有什么区别？
Opened this issue · 3 comments
ultraypy commented
我可不可以自己对raw matrix进行预处理和过滤
lishuangshuang0616 commented
Raw is the transcript expression matrix of all detected cell barcodes,
and filter is the matrix identified as real cells after the background is filtered out by cell calling.
It is recommended to use filter, you can also use raw to filter manually.
ultraypy commented
Can I use the Seurat workflow for filtering?
lishuangshuang0616 commented
yes , use subset to filter ,subset gene,umi,mt etc
pbmc <- [subset](https://rdrr.io/r/base/subset.html)(pbmc, subset = nFeature_RNA > $num1 & nFeature_RNA < $num2 & percent.mt < $num3)