/fpm

Fourier Ptychographic Microscopy Simulation in MATLAB

Primary LanguageMATLAB

Fourier Ptychographic Microscopy (FPM) Simulation

Implementation of a simulation of FPM as described by Zheng et al in their paper [1]. In the supplmentary information of this paper, a simulation of FPM has been described which I will be copying almost exactly, using inbuilt MATLAB images as the authors have done. The demo video of this can be seen at https://www.youtube.com/watch?v=gnFBuvy0bh4

Image generation and explanation in brief

The first part tackled shall be 'generation' of images for FPM - i.e. using a simulated object with known intensity and phase profiles, it shall be 'illuminated' at different known angles by a monochromatic light source (632 nm) to give a greyscale image at the output representing the obliquely illumation object. The original object shall have dimensions 256x256 pixels and each pixel is assumed to be of 275nm dimension - this is important in the plane wave calculation.

The obliquely illumated intensity images will be sampled at 5.5 micron pixel width (i.e. 20 times less resolution) and these low-res images will be the end point of the image generation part.

Image reconstruction

The generated images will be stitched together in fourier space as described in the publication referred to earlier to get back the original high-resolution image as well as the phase information that is hidden in the complex portion of the sampled image. The reconstruction of the high-res object as well as phase image shall be taken as onfirmation that the algorithm written for FPM reconstruction works. This code will be used as-is (after re-writing in python and subsequently in C++) for reconstruction of images in the real world through microscopy.

References

[1] http://dx.doi.org/10.1038/nphoton.2013.187