Pinned Repositories
201912-12-entity-sentiment-analysis-reddit
Final project code for Timotius Kartawjiaya, Fernando Troeman, and Hritik Jain
abstreet
A traffic simulation game exploring how small changes to roads affect cyclists, transit users, pedestrians, and drivers.
OpenDiablo2
An open source re-implementation of Diablo 2
rlcard
Reinforcement Learning / AI Bots in Card (Poker) Games - Blackjack, Leduc, Texas, DouDizhu, Mahjong, UNO.
MyOneTaps's Repositories
MyOneTaps/api
Community discussion and documentation for the NWS API
MyOneTaps/arenamaster-addon
ArenaMaster.IO PvP Inspect AddOn
MyOneTaps/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
MyOneTaps/BlizzHUDTweaks
MyOneTaps/Block-Site
A browser extension to block website access using hostname matching
MyOneTaps/deskreen
Deskreen turns any device with a web browser to a second screen for your computer
MyOneTaps/Diminish
[WoW] Diminishing returns tracker for players crowd controls.
MyOneTaps/Factor_Launcher_Reboot
The new repository for Factor Launcher, inspired by Windows Phone 7
MyOneTaps/frame-sort
A simple WoW add-on that places the player frame (you) at the top or bottom (configurable) of the party/raid frames.
MyOneTaps/icer_compression
Progressive, error tolerant, wavelet-based image compression algorithm
MyOneTaps/input-leap
Open-source KVM software
MyOneTaps/passbolt_api
Passbolt CE Backend, a JSON API written with Cakephp
MyOneTaps/pikvm
Open and inexpensive DIY IP-KVM based on Raspberry Pi
MyOneTaps/pingfs
Stores your data in ICMP ping packets
MyOneTaps/plugins
Joplin official plugin repository
MyOneTaps/pvpleaderboard
View and filter arena and RBG leaderboards. See which talents top World of Warcraft PvPers are selecting.
MyOneTaps/pvpleaderboardupdater
Updates the pvpleaderboard database
MyOneTaps/Remotely
A remote control and remote scripting solution, built with .NET Core, SignalR Core, and WebRTC.
MyOneTaps/return-youtube-dislike
Chrome extension to return youtube dislikes
MyOneTaps/SortGroup
MyOneTaps/SquadOVNext
MyOneTaps/STRIKER
Record CS:GO highlights from the comfort of Discord.
MyOneTaps/Surfingkeys
Map your keys for web surfing, expand your browser with javascript and keyboard.
MyOneTaps/tenacity
FLOSS Audacity Fork · No telemetry, crash reports and other shenanigans like that!
MyOneTaps/The-One-With-The-Analysis
In the repository, I analyse all the episodes from the popular TV show Friends. I look mainly at sentiment analysis, and try to draw correlations with viewership, ratings and other parameters.
MyOneTaps/twitch-dvr
A chrome extension to enable DVR on Twitch streams.
MyOneTaps/TwitchAdSolutions
MyOneTaps/wifi-location-changer
automatically change location when mac os x changes wifi connection
MyOneTaps/wow-ui-source
git mirror of the user interface source code for World of Warcraft
MyOneTaps/zero