ANAPURRNAseq : Automated Nexflow Alignement Pipeline for Uncleared Reads from RNAseq
#Bioinformatics #pipeline
Tasks
- Test the pipeline on the a dataset in Data/Sample_dataset
- adapt the code to reduce space usage at the rRNA step
- test on Data/Sample_dataset
- README
- Usage
- Step description
- introduction
- publish the pipeline on Github
Done
- Create a pipeline using NextFlow The pipeline can be found into /Bioinformatics/ReadCountPipeline
- download all files
- generate fastQC reports
- generate MultiQC report
- sort reads using Clumpify
- Remove adapters
- Quality filtring
- Artifact filtering
- Contamination removal
- filtering for low complexity
- Remove rRNA
- QC Analysis