Found peptides are not matching protein sequences
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LifeWorks commented
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<style> </style>| Peptide Sequence | Prev AA | Next AA | Start | End | Peptide Length | Charges | Protein | Protein ID |
|---|---|---|---|---|---|---|---|---|
| EIIEQIR | R | N | 154 | 160 | 7 | 2 | sp|P31948|STIP1_HUMAN | P31948 |
| CVIGGDGSLTGANIFR | L | K | 123 | 138 | 16 | 2 | sp|Q01813|PFKAP_HUMAN | Q01813 |
| GITNLCVIGGDGSLTGANIFR | R | K | 118 | 138 | 21 | 2,3 | sp|Q01813|PFKAP_HUMAN | Q01813 |
| ILPLVVK | K | F | 256 | 262 | 7 | 2 | sp|Q86VP6|CAND1_HUMAN | Q86VP6 |
| SVLLSLK | R | Q | 236 | 242 | 7 | 2 | sp|P09496|CLCA_HUMAN | P09496 |
| QIIDEAER | Q | S | 381 | 388 | 8 | 2 | sp|P78371|TCPB_HUMAN | P78371 |
| SLHDALCVI | R | A | 389 | 397 | 9 | 2 | sp|P78371|TCPB_HUMAN | P78371 |
| IIGELAK | R | E | 79 | 85 | 7 | 2 | sp|Q99584|S10AD_HUMAN | Q99584 |
| FRDSLLR | R | S | 516 | 522 | 7 | 2,3 | sp|P49327|FAS_HUMAN | P49327 |
| LLEQGIR | K | H | 437 | 443 | 7 | 2 | sp|P49327|FAS_HUMAN | P49327 |
| AGALEGDLFIGPK | M | A | 49 | 61 | 13 | 2 | sp|P61163|ACTZ_HUMAN | P61163 |
| SHLEILR | K | G | 165 | 171 | 7 | 2 | sp|P00390|GSHR_HUMAN | P00390 |
| AELELQK | K | D | 108 | 114 | 7 | 2 | sp|P07741|APT_HUMAN | P07741 |
| QIHDLVLVGGSTR | S | I | 330 | 342 | 13 | 3 | sp|P11142|HSP7C_HUMAN | P11142 |
| MPLIGLGTWK | K | S | 13 | 22 | 10 | 2 | sp|P15121|ALDR_HUMAN | P15121 |
| PLIGLGTWK | M | S | 14 | 22 | 9 | 2 | sp|P15121|ALDR_HUMAN | P15121 |
| ELIGTCK | R | M | 320 | 326 | 7 | 2 | sp|P18206|VINC_HUMAN | P18206 |
| IIGAVAK | R | V | 824 | 830 | 7 | 2 | sp|P18206|VINC_HUMAN | P18206 |
| SLLYDER | K | S | 54 | 60 | 7 | 2 | sp|P25787|PSA2_HUMAN | P25787 |
| GIATVLR | K | Y | 458 | 464 | 7 | 2 | sp|Q7KZF4|SND1_HUMAN | Q7KZF4 |
| AAVPSLK | K | F | 42 | 48 | 7 | 2 | sp|P00558|PGK1_HUMAN | P00558 |
| ALLDETK | Q | G | 326 | 332 | 7 | 2 | sp|P04083|ANXA1_HUMAN | P04083 |
| CLTALVK | K | C | 263 | 269 | 7 | 2 | sp|P04083|ANXA1_HUMAN | P04083 |
| LGVTVLSR | K | I | 199 | 206 | 8 | 2 | sp|P16152|CBR1_HUMAN | P16152 |
| ADTPEIQR | V | I | 102 | 109 | 8 | 2 | sp|Q14847|LASP1_HUMAN | Q14847 |
| ALILIPK | D | V | 443 | 449 | 7 | 2 | sp|P40227|TCPZ_HUMAN | P40227 |
There are around 135 from ~18000 peptides are not matching their protein sequences.
danielgeiszler commented
@fcyu I checked the first one, it's a I/L flattening issue
fcyu commented
It is due to the I and L interchange. When Philosopher matched the peptides to proteins, it does not distinguish I and L because they have the identical mass.
Best,
Fengchao