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Folder information
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Usage of DIPins
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Output
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Platform
When you download the DIPins software package, you will see that there are four directories.
1.DIPins
This directory contains the DIPins program.
2.example
This directory contains example files, including two pair of fastq files and a reference sequence file.
3.run
This directory contains the running script and jar. If you want to do the insertion detection in Gui,you can run runGui.sh or java -jar DIPinsGui.jar in run directory to Start a Gui. If you want to do the insertion detection in Terminal,you can run runTerminal.sh.
4.software
This directory contains two software that depends on it.
2.1 how to use in Gui
You can directly run it by java -jar DIPinsGui.jar in run directory
In Gui：
(1)FASTA File：the input fast file（eg:*.fa or *.fasta）
(2)FASTQ File1：the input fastq file 1(eg:*.fq or *.fastq)
(3)FASTQ File2：the input fastq file 2(eg:*.fq or *.fastq)
(4)Output Directory：the output directory.The resulting files will be placed in this folder(insert_result.txt).
(5)Cache Directory：temp directory for DIPins running
(6)BWA Directory：Third-party software bwa folder
(7)Seqtk Directory：Third-party software seqtk folder
(8)Chr：the detection chr name（eg：chr21）
insertion_result.txt has a total of five columns
(1)：reference sequence name
(2)：the position where the mutation occurred
(3)：the length of mutation
(4)：Sequence information without mutation
(5)：Sequence information with mutation
DIPins runs on a Linux system