[question] Auxotroph E. coli

[emarquezz]

Hi!,

I'm trying to model a E. coli that has mutations that make it auxotroph for some aminoacids. I added the mutations on the genes I am interested but I think I might be doing something wrong when I try to make E. coli consume the aminoacids that it's lacking.I tried to make it consume them by applying a negative value on the "EX_" reactions of each aminoacid but the model won't be solved. Do you have any ideas on what am I missing?

Thank you!