SEVA77's Stars
beyondcode/expose
A beautiful, fully open-source, tunneling service - written in pure PHP
antoniomika/sish
HTTP(S)/WS(S)/TCP Tunnels to localhost using only SSH.
cyberspacedk/Git-commands
Шпаргалка по консольным командам Git
00-Evan/shattered-pixel-dungeon
Shattered Pixel Dungeon is an open-source traditional roguelike dungeon crawler with randomized levels and enemies, and hundreds of items to collect and use. It's based on the source code of Pixel Dungeon, by Watabou.
MFDESTINO/attiny13_spi
Very simple bitbanged SPI for Attiny13A
rkl099/Appinventor-SerialOTG
Appinventor extension SerialOTG with BT/WIFI client/server support, examples of apps and Arduino code, ver 1.5
you-dont-need/You-Dont-Need-JavaScript
CSS is powerful, you can do a lot of things without JS.
HeaTTheatR/KivyMD-data
jacklinquan/usbserial4a
Python package for Android USB host serial port.
rms-support-letter/rms-support-letter.github.io
An open letter in support of Richard Matthew Stallman being reinstated by the Free Software Foundation
NAalytics/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
Swordfish90/cool-retro-term
A good looking terminal emulator which mimics the old cathode display...
caiyongji/emoji-list
emoji list; emoji表情列表
twitter/twemoji
Emoji for everyone. https://twemoji.twitter.com/
movim/movim
Movim - Decentralized social platform
iv-org/invidious
Invidious is an alternative front-end to YouTube
FreeTubeApp/FreeTube
An Open Source YouTube app for privacy
motioneye-project/motioneyeos
A Video Surveillance OS For Single-board Computers
micasa-acerman/portfolio
Portfolio site on pure html5 and css3
seawarrior181/PasswordPump
An ATMega32u4 USB based credentials manager
commentics/commentics
Commentics - A free PHP comment script
digitalsparky/jekyll-minifier
Jekyll HTML/XML/CSS/JS Minifier utilising yui-compressor, and htmlcompressor
solemnwarning/rehex
Reverse Engineers' Hex Editor
Ultimate-Hosts-Blacklist/Ultimate.Hosts.Blacklist
The Ultimate Unified Hosts file for protecting your network, computer, smartphones and Wi-Fi devices against millions of bad web sites. Protect your children and family from gaining access to bad web sites and protect your devices and pc from being infected with Malware or Ransomware.
mrtkp9993/VHDLExamples
VHDL examples.
monero-project/monero
Monero: the secure, private, untraceable cryptocurrency
digao-dalpiaz/Dam
Delphi and Lazarus Message Dialogs with Formatted Text
responsively-org/responsively-app
A modified web browser that helps in responsive web development. A web developer's must have dev-tool.
al177/esp8089
Linux kernel module driver for the ESP8089 WiFi chip
taylorconor/quinesnake
A quine that plays snake over its own source!