SysBioChalmers/Sco-GEM

feat: include cofactor information from Coze et al., 2013

sulheim opened this issue · 0 comments

From Coze et al., 2013 we have: We found that glucose-6-phosphate dehydrogenase was strictly NADP-dependent, with a specific activity of 144.662.7 mmol/min/mg protein and no detectable activity with NAD. Furthermore, we found that 6-phosphogluconate dehydrogenase was strictly NADP-dependent, with a specific activity of 236.466.4 mmol/min/mg protein and no detectable activity with NAD. We also verified that isocitrate dehydrogenase was strictly NADP-dependent, with a specific activity of 132.764.3 mmol/min/mg protein and no activity at all with NAD.

Therefore, reactions with the wrong cofactors should be deleted, and this can probably be mentioned in the note field of the correct cofactor-related reaction and with a doi annotation to Coze et al., 2013, in the annotation field.
The following reactions should then be deleted:

  • G6PDH1b
  • GND2

To do:

  • Incorporate changes into reconstruction script and
  • Reconstruct xml and yml model files

I hereby confirm that:

  • This problem persists in the master branch of the repository
  • A similar issue does not already exist
  • I've considered asking this first in the Gitter chat room