Make read trimming optional
TBradley27 opened this issue · 0 comments
TBradley27 commented
There are some studies which demonstrate that trimming of RNA-Seq reads does not have a substantial impact on the accuracy of the quantification of gene expression. For example, the following preprint:
https://www.biorxiv.org/content/10.1101/833962v1.abstract
Removing trimming from the workflow would substantially reduce overall workflow runtime.
Therefore, it would be beneficial if trimming in FilTar could be made optional at this stage, but made the default option nonetheless (until more thoughtful consideration is given to this topic by myself).