I'm designing species specific PCR primers for identification of the three bacteria Alvar Almsted has worked with in this project.
Data is located in /nobackup/data6/Primer_design_bacteria on Albiorix.
| Genome | Primer name | Sequence | Length | %GC | Tm °C | Frag. length |
|---|---|---|---|---|---|---|
| unitig_0 | U0V1fw | GAG AAA GCA CTT CGG TGT GAG | 21 | 52 | 54.4 | 1222 |
| unitig_0 | U0V8rev | GAG CGG TTT TAT GGG ATT CGC | 21 | 52 | 54.4 | 1222 |
| unitig_1 & 2 | KSV2fw | CCT CAT AGT ACT GTT GAA TCG CC | 23 | 48 | 55.3 | 669 |
| unitig_1 & 2 | KSV5rev | GAC CAA AGT CCA AAC AGC TAG | 21 | 48 | 52.4 | 669 |
| genome_4 | G4V1fw | CAA GTC GAA CGA GAA GCT AC | 20 | 50 | 51.8 | 1335 |
| genome_4 | G4V9rev | CAC CTT CGG GTA AAG CCA AC | 20 | 55 | 53.8 | 1335 |