TheOnlyArtz's Stars
denoland/deno
A modern runtime for JavaScript and TypeScript.
oven-sh/bun
Incredibly fast JavaScript runtime, bundler, test runner, and package manager – all in one
juliangarnier/anime
JavaScript animation engine
iovisor/bcc
BCC - Tools for BPF-based Linux IO analysis, networking, monitoring, and more
dolthub/dolt
Dolt – Git for Data
deviantony/docker-elk
The Elastic stack (ELK) powered by Docker and Compose.
dogecoin/dogecoin
very currency
mozilla/sccache
Sccache is a ccache-like tool. It is used as a compiler wrapper and avoids compilation when possible. Sccache has the capability to utilize caching in remote storage environments, including various cloud storage options, or alternatively, in local storage.
kemalcr/kemal
Fast, Effective, Simple Web Framework
NAalytics/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
kkkgo/KMS_VL_ALL
🔑KMS_VL_ALL - Smart Activation Script
Amanieu/parking_lot
Compact and efficient synchronization primitives for Rust. Also provides an API for creating custom synchronization primitives.
foniod/redbpf
Rust library for building and running BPF/eBPF modules
alshdavid/BorrowScript
TypeScript with a Borrow Checker. Multi-threaded, Tiny binaries. No GC. Easy to write.
marcelja/facebook-delete
Fast facebook activity deletion
dan200/ComputerCraft
Programmable Computers for Minecraft
celo-org/celo-blockchain
Official repository for the golang Celo Blockchain
jonhoo/rust-tcp
A learning experience in implementing TCP in Rust
Kudaes/DInvoke_rs
Dynamically invoke arbitrary unmanaged code
ropensci/crul
R6 based http client for R (for developers)
OGoodness/Minesweeper-Login
Minesweeper that cannot be exited in any way other than completing the game. Make it run while logging in and it will work as a second form of "auth". Used as a joke for Cyber Team --- Bypass with code - 6969420
dariushhpg1/IconaMoon
+2400 icons in 8 styles. Available for: Figma, Sketch, Adobe XD, SVG, PNG. by Dariush Habibpour
exploitblizzard/WindowsMDM-LPE-0Day
CVE-2021-24084 Windows Local Privilege Escalation Left officially unpatched since 2020. Hence, its still a zero day
t5mat/turnbinds
Turnbinds (+left/+right) with customizable yawspeed for CS:GO
kingsleyh/crystal-rethinkdb
driver for rethinkdb / rebirthdb
TheOnlyArtz/rust-udp-holepunch
A small holepunching implementation written in Rust (UDP)
YakirOren/sratim
Download movies from sratim.tv
amitka-rnd/rust_cmd_lib_cf
THIS ONE SUPPORTS CREATION_FLAGS
TheOnlyArtz/imperialshag
A multi-platform distributed C&C written purely in Rust
TheOnlyArtz/rustirc
A fully asynchronous IRC client (and protocol implementation) written in Rust