EUN is the website provides the easy way to analyze CRISPR/Cas efficiency.
You can analysis CRISPR/Cas with more than one sequencing data (paired-end reads). Also, can run even more than 100 .gz file.
If you want to test analysis, just click 'Run analysis sample file'.
You can also analyze CRISPR/Cas by the json file in one seconds. Just download json file after first analyzed CRISPR/Cas and click 'Analyze by json file'.
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File Name Pattern (Optional) This is necessary to identify the gz file. If you omit this box, unique id will be the text before the 'File Index Pattern'. eg. File Name: Sample-ID-86S86_L001_R1_001.fastq.gz File Name Pattern: Sample-ID File Index Pattern: _L001 File ID: 86_S86
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File Index Pattern Required for indexing the files.
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Sequencing Data (Paired-end reads) Input files for analyze it. Should be gz file for analyze CRISPR/Cas.
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Reference Amplicon Sequence Reference Amplicon Sequence
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Used Cas otholog EUN only provides NGG types, but someday more will be added for analysis.
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Target DNA sequence (5 to 3, without PAM sequence) Target DNA sequence
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Standard Range Standard target range
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Target Nucleotide Input the unchanged nucleotide type.
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Desired change of target nucleotide Input the changed nucleotide type.
Shows the average of insertion and deletion as a line chart. You need the json file downloaded after CRISPR/Cas analysis. Also could download the chart by png file.
You can convert the file or change the sequence data. Click the button what you want to convert and after convert it would be downloaded for zip file.
- Reverse sequence It makes reverse the original and changed sequence.
- Reverse nucleotide It makes reverse the nucleotide A to T, G to C.
- Merge files Get the analyzed file or INDEL chart in one file.