Motivation: Single-cell RNA sequencing (scRNA-seq) technology has revolutionized the way research is done in biomedical sciences. It provides an unprecedented level of resolution across individual cells for studying cell heterogeneity and gene expression variability. Analyzing scRNA-seq data is challenging though, due to the sparsity and high dimensionality of the data.
Results: I developed scGEAToolbox—a Matlab toolbox for scRNA-seq data analysis. It contains a comprehensive set of functions for data normalization, feature selection, batch correction, imputation, cell clustering, trajectory/pseudotime analysis, and network construction, which can be combined and integrated to building custom workflow. While most of the functions are implemented in native Matlab, wrapper functions are provided to allow users to call the “third-party” tools developed in Matlab or other languages. Furthermore, scGEAToolbox is equipped with sophisticated graphical user interfaces (GUIs) generated with App Designer, making it an easy-to-use application for quick data processing.
disp('Install scGEAToolbox...')
unzip('https://github.com/jamesjcai/scGEAToolbox/archive/main.zip');
addpath('./scGEAToolbox-main');SCGEATOOL is a standalone application running on Windows machines that do not have MATLAB installed. SCGEATOOL is a lightweight and blazing fast desktop application that provides interactive visualization functionality to analyze single-cell transcriptomic data. SCGEATOOL allows you to easily interrogate different views of your scRNA-seq data to quickly gain insights into the underlying biology. SCGEATOOL is a pre-compiled standalone application developed in MATLAB. Pre-compiled standalone releases are meant for those environments without access to MATLAB licenses. Standalone releases provide access to all of the functionality of the SCGEATOOL standard MATLAB release encapsulated in a single application. SCGEATOOL is open-sourced to allow you to experience the added flexibility and speed of the MATLAB environment when needed.
If you have any questions or require assistance using scGEAToolbox, please contact me.
Cai JJ. scGEAToolbox: a Matlab toolbox for single-cell RNA sequencing data analysis. Bioinformatics. 2019;btz830. doi:10.1093/bioinformatics/btz830