/RNPC3_ONT

RNPC3 knockdown A549 cell line long- and short-read RNA-seq analysis

Primary LanguageR

Using long- and short-read RNA-seq to study minor class intron splicing

This repository contains code used to perform the analysis and generate the figures for my PhD thesis chapter 4.

Files and folders

aligned_minimap2: genomic alignment BAM files and scripts for mapping

bambu: scripts and outputs for bambu

salmon: scripts and outputs for salmon

short_bam_merged: merge bams of samples from the same group for visualization of coverage

transcript_analysis: scripts and outputs for transcriptomic analysis: quality control, DTE, DTU, visualization of read coverage

DTE and DTU results table

These can be found in transcript_analysis/results (for long read) and transcript_analysis/results_short (for short read).