Pipeline for BLAST analysis of IonTorrent DNA barcoding data.
requirements: Linux
dependencies: python-biopython (RPM)
This pipeline takes FastQ input files and converts them to FASTA format to be used in our BLAST section of the script. The FASTA file will then be blasted (blastn) and results are passed back as an XML file. The XML file is then used to perform a quality control (parameters will be entered by the user) and displayed back to the user's terminal. The results will then be checked on the CITES list (available at http://www.cites.org/eng/app/appendices.php) to see if there are any illegal "hits". The results are displayed to the user on the command line