ask for the minimum bam file numbers

[zwyy5520]

Hello,

if I only have 4 bam files , can I continue to use scDapars for analysis?
when I run scDapars with 4 bam files ,the program reported the following info :

---

[1] "Reading in raw PDUI matrix ..."
[1] "number of genes in raw count matrix 2353"
[1] "number of cells in raw count matrix 8"
[1] "Reading finished!"
[1] "Start Processing raw PDUI matrix"
[1] "Pre-Processing finished"
[1] "Start Imputation Steps ..."
[1] "Find potential Neighboring Cells ..."
Error in RANN::nn2(t(mat_pcs), k = 30) :
Cannot find more nearest neighbours than there are points

So,what changes should I make to let this program work?
Looking forward to your reply. Thank you.

[YiPeng-Gao]

since scDaPars uses clustering first to find neighboring cell, if you only have 4 samples, it doesn't make sense to run scDaPars. You will need to create 1 bam file per cell to run scDaPars.