Exp337CD25KONascent
Contributors:
Huitian Diao (Yolanda): Experiment & Binformatics
Dapeng Wang: Experiment
Matthew Pipkin: Advisor
Manuscript progress
External data storage
Experiment design
- Cell types: P14 CD25KO v.s. P14 WT
- Time points (Post in vitro activation): 0h, 6h, 24h, 48h
Analysis steps
0. QC
Output files: Multi-QC results
1. Trim & Alignment & Convert & Filter
Script: HPC codes
2. Strand split & HTseq count & Count compilation & Normalization
Script: HPC codes
Output files:
HTseq-count output
Compiled count
Normalized TPM
3. DEseq2
Script: DEseq2
Output files:
DEseq2 output with ENSEMBL-ID
DEseq2 output with GeneName