aloncarmel

aloncarmel.github.io

My homepage

bootlander

Bootlander default landing page

devunityatom

Devunity atom.io plugin

firecam

Firecam lets you broadcast simple video using your html5 canvas camera using firebase to the internet. a very simple solution for broadcasting lagging video without any servers.

flipit4diggreader

Add Flipboard Flip-it sharing button to your digg reader!

multiplayerjs

A Multiplayer JS Library using Firebase to add Figma/Miro like multiplayer experience to any web page in real time

ruckuscaptiveportal

External self hosted Ruckus Smart Zone Captive Portal using Ruckus Hotspot external URL

wilcosdk

Wilco SDK and Editor documentation

aloncarmel/devunityatom

Devunity atom.io plugin

aloncarmel/multiplayerjs

A Multiplayer JS Library using Firebase to add Figma/Miro like multiplayer experience to any web page in real time

aloncarmel/affinitycojs

A Javascript Wrapper Library for Accessing Affinity.co api

aloncarmel/ai-collection

The Generative AI Landscape - A Collection of Awesome Generative AI Applications

aloncarmel/AI-Infinity

A set of AI tools that will help you explore the infinite possibilities of AI.

aloncarmel/AI-Toolbox

Building a comprehensive resource for AI enthusiasts and professionals

aloncarmel/aloncarmel

aloncarmel/Anythink-Market-cc4ax

aloncarmel/Anythink-test

aloncarmel/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273

RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.

aloncarmel/Awesome-AI

A curated list of awesome things related to artificial intelligence tools around the world wide web

aloncarmel/awesome-ai-list-guide

The guide of awesome list about AI

aloncarmel/awesome-ai-tools

Finding the AI tools you need!

aloncarmel/Awesome-AITools

Collection of AI-related utilities. Welcome to submit issues and pull requests /收藏AI相关的实用工具，欢迎提交issues 或者pull requests

aloncarmel/awesome-generative-ai

A curated list of modern Generative Artificial Intelligence projects and services

aloncarmel/awesome-generative-ai-1

A curated list of Generative AI tools, works, models, and references

aloncarmel/awesome-generative-ai-2

An awesome list of low- and no-code generative AI resources.

aloncarmel/awesome-generative-ai-companies

A curated list of Gеnerative AI companies, sorted by focus area and total fundraised amount.

aloncarmel/epideme

Epidemiology investigation framework for creating a personal report of where you've been, who you've met and when.

aloncarmel/firepad

Collaborative Text Editor Powered by Firebase

aloncarmel/free-for-dev

A list of SaaS, PaaS and IaaS offerings that have free tiers of interest to devops and infradev

aloncarmel/neko

A self hosted virtual browser that runs in docker and uses WebRTC.

aloncarmel/quest-template

aloncarmel/react

A declarative, efficient, and flexible JavaScript library for building user interfaces.

aloncarmel/skills-copilot-codespaces-vscode

My clone repository

aloncarmel/skills-introduction-to-github

My clone repository

aloncarmel/testrepo

aloncarmel/Top-AI-Tools

Curated list of AI Tools

aloncarmel/vscode-firebase

Synchronize VSCode editor content to Firebase

aloncarmel/wilco-docs