Program used to calculate the colocalization between three color channels during immunofluoresence micoscopy. Program will calculate the percentage of overlap of channel 1 to both channels 2 and 3 together. Output colocalization is based on Manders' Colocalization Coefficient. To use this file:
- External libraries needed: PIL, xlwt, xlrd, xlutils
- Create an empty folder anywhere on computer.
- Input your three individual color channel images into the folder.
- Start the file and, when prompted, select the folder that contains the three images to analyze.
- Adjust the image brightness as necessary with slider bar and press Update button.
- Press Analyze button.
- Excel sheet should autogenerate in Colocalization folder with mM1, mM2, and mM3 coefficients.