unhashable type: 'Series'

[LJZYaaa]

Hi! I'm very interesting in your powerful tool, and trying to apply it to my own data. But when i run the code in your tutolial :
fs.pp.construct_flows_from_cellchat(adata,
cellchat_output_key,
gem_expr_key = 'X_gem',
scale_gem_expr = True,
model_organism = 'human',
flowsig_network_key = 'flowsig_network',
flowsig_expr_key = 'X_flow')
it report that:

what's more, when i skip this code and try the next one , it en even report that：

is the version in your tutotial not the same as it in the repositort? and how to update it?
Looking forward to your reply, and hope anything goes well on you! ^^

[LJZYaaa]

@axelalmet

[axelalmet]

Hi,

Thanks for your question! To respond to each of your questions:

1. It looks like you're running an older version of FlowSig. Could you try updating to the latest version in the GitHub repository and running fs.pp.construct_Flows_from_cellchat again?

2. You're quite right, the tutorial to construct differentially flowing variables in the README.md is out of date. Apologies for this mistake! Instead of running fs.pp.determine_informative_variables, please run:

fs.pp.determine_differentially_flowing_vars(adata,
                                        condition_key = condition_key,
                                        control_key = 'CON',
                                        flowsig_expr_key = 'X_flow',
                                        flowsig_network_key = 'flowsig_network',
                                        qval_threshold = 0.05,
                                        logfc_threshold = 0.5)

I've updated the README.md to reflect this.

Hope these help!

[LJZYaaa]

Hi, i do install flowsig again, and the fs.pp.construct_flows_from_cellchat() don't report error anymore. But when i try to run the command fs.pp.determine_differentially_flowing_vars() as your reply, it says that:

maybe the version in your repositort is not the same as in your labs?
Looking forward to your reply, hope anything goes well on you~~ ^^

[LJZYaaa]

Another question is that how can we visulize the result as it in your paper? :)

[yixudong]

Hi, i do install flowsig again, and the fs.pp.construct_flows_from_cellchat() don't report error anymore. But when i try to run the command fs.pp.determine_differentially_flowing_vars() as your reply, it says that: maybe the version in your repositort is not the same as in your labs? Looking forward to your reply, hope anything goes well on you~~ ^^

Hi, LJZYaaa, I'm having the same issue.

Have you solved this problem now?

[yixudong]

Another question is that how can we visulize the result as it in your paper? :)

@axelalmet

[LJZYaaa]

Not yet :) the author has been inactive in some time :)