/MINPP1_reactionNetwork

Nguyen Trung, M., Kieninger, S., ... B. G. Keller, Fiedler, D. (2022). "Stable isotopomers of myo-inositol to uncover the complex MINPP1-dependent inositol phosphate network", ACS Central Science, 8(12), 1683-1694. https://doi.org/10.1021/acscentsci.2c01032

Primary LanguagePython

MINPP1_reactionNetwork

this repository contains supporting information to the manuscript:
Nguyen Trung, M., Kieninger, S., ... B. G. Keller, Fiedler, D. (2022). "Stable isotopomers of myo-inositol to uncover the complex MINPP1-dependent inositol phosphate network", ACS Central Science, 8(12), 1683-1694.
https://doi.org/10.1021/acscentsci.2c01032

In our work, we numerically analyzed experimentally determined progress curves for the MINPP1 mediated dephosphorylation of InsP5[2OH] and InsP6. This repository contains the corresponding Python3 scripts that were used to extract the kinetic rates from the experimental data sets.



Overview

All analysis was performed with Python3. The files "fitted_exp_data.py" contain all fit functions that where used to fit the scaled experimental data. The files "minimization_routine.py"contain the code for the entire minimization routine including initial guess and reconstruction of the progress curves from the evaluated rates. The directory tree is as follows:

Code
  |
  ├── InsP5[2OH]
  |       |   fitted_exp_data.py
  |       |   minimization_routine.py
  |
  |      
  └── InsP6
          |   fitted_exp_data.py
          |   minimization_routine.py