Fred Hutchinson Cancer Research Center, Seattle, WA
6-7 April, 2015
Contact: Martin Morgan (mtmorgan@fredhutch.org)
This INTERMEDIATE course is designed for individuals comfortable using R, and with some familiarity with Bioconductor. It consists of approximately equal parts lecture and practical sessions addressing use of Bioconductor software for analysis and comprehension of high-throughput sequence and related data. Specific topics include use of central Bioconductor classes (e.g., GRanges, SummarizedExperiment), RNASeq gene differential expression, ChIP-seq and methylation work flows, approaches to management and integrative analysis of diverse high-throughput data types, and strategies for working with large data. Participants are required to bring a laptop with wireless internet access and a modern version of the Chrome or Safari web browser.
Please register online.
Day 1 (9:00 - 12:30; 1:30 - 5:00)
- A. Introduction. Bioconductor and sequencing work flows
- B. Genomic Ranges. Working with Genomic Ranges and other Bioconductor data structures (e.g., in the GenomicRanges. package).
- C. Differential Gene Expression. RNA-Seq known gene differential expression with DESeq2 and edgeR.
Day 2 (9:00 - 12:30; 1:30 - 5:00)
- D. Machine Learning.
- E. Gene Set Enrichment.
- F. ChIP-seq ChIP-seq with csaw
- I. Large Data -- efficient, parallel, and cloud programming with BiocParallel, GenomicFiles, and other resources.