Pinned Repositories
Active-Directory-Exploitation-Cheat-Sheet
A cheat sheet that contains common enumeration and attack methods for Windows Active Directory.
Active-Directory-ExploitCS
A cheat sheet that contains common enumeration and attack methods for Windows Active Directory.
Active_Directory_Advanced_Threat_Hunting
This repo is about Active Directory Advanced Threat Hunting
Active_Directory_with_Windows_Server_2022
Everything about Active Directory in a hybrid infrastructure!
ad-password-protection
Active Directory password filter featuring breached password checking and custom complexity rules
adsec
An introduction to Active Directory security
adversary_emulation_library
An open library of adversary emulation plans designed to empower organizations to test their defenses based on real-world TTPs.
AggressorScripts
Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
Skiptrace
Sites and tools to find people
brittadams's Repositories
brittadams/Skiptrace
Sites and tools to find people
brittadams/awesome-selfhosted
This is a list of Free Software network services and web applications which can be hosted locally. Selfhosting is the process of locally hosting and managing applications instead of renting from SaaS providers.
brittadams/awesome-windows-domain-hardening
A curated list of awesome Security Hardening techniques for Windows.
brittadams/cpbitmap2pgn
Convert apple cpbitmap image to pgn
brittadams/esp8266_deauther
Scan for WiFi devices, block selected connections, create dozens of networks and confuse WiFi scanners!
brittadams/Fortigate-password-recovery
Fortigate password recovery
brittadams/fosscad-repo
Official FOSSCAD Library Repository
brittadams/Graylog_Sysmon
Advanced configuration for Graylog w/Sysmon
brittadams/iDRACula
iDRACula uses the Shodan API (www.shodanhq.com) to search for Dell iDRAC boards exposed to the internet with default credentials
brittadams/NETworkManager
A powerful tool for managing networks and troubleshoot network problems!
brittadams/O365-Admin-Center
The O365 Admin Center is a GUI application that administrators can use to administer every aspect of Office 365 including Exchange Online, Compliance Center, SharePoint and Skype for Business.
brittadams/Office365
Office 365 scripts and information
brittadams/OfficeDeployFramework
A batch script to demonstrate complex Microsoft Office deployments
brittadams/sentinel-attack
Repository of sentinel alerts and hunting queries leveraging sysmon and the MITRE ATT&CK framework
brittadams/SwiftFilter
Exchange Transport rules to detect and enable response to phishing
brittadams/sysmon-config
Advanced Sysmon configuration, Installer & Auto Updater with high-quality event tracing
brittadams/the-book-of-secret-knowledge
A collection of inspiring lists, manuals, cheatsheets, blogs, hacks, one-liners, cli/web tools and more.
brittadams/ThreatHunter-Playbook
A Threat hunter's playbook to aid the development of techniques and hypothesis for hunting campaigns.
brittadams/WinAdminCenterPS
Copy of Windows Admin Center (https://docs.microsoft.com/en-us/windows-server/manage/windows-admin-center/overview) PowerShell Functions (as of 7-26-2018).