A Python script to convert the spectral library in BLIB format (.blib) from either Skyline or BiblioSpec to readable library format (OpenSwath or Spectronaut tsv format)
>pip install -r requirements.txt
>python blib2openswath.py -h
usage: blib2openswath.py [-h] [--infile -i [-i ...]] [--fasta -f [-f ...]] [--tol -t [-t ...]] [--mz_type -m [-m ...]]
Blib2OpenSwath: Convert spectral library from BLIB (Skyline) to OpenSwath format
optional arguments:
-h, --help show this help message and exit
--infile -i [-i ...] The input spectral library file in BLIB format either from Skyline or BiblioSpec output
--fasta -f [-f ...] Proteome database in fasta format for mapping peptides sequences
--tol -t [-t ...] Library match tolerance in dalton (Da) for fragment m/z annotation (INFO: The tolerance of 0.5 Da and 0.05 Da was set as default)
--mz_type -m [-m ...] Specify the type of fragment m/z values present in the input spectral library (Ex: "average" or "mono")
--lib_fmt -l [-l ...] Specify the spectra library format (Ex: "openswath" or "spectronaut")
--fmt -o [-o ...] Specify the output spectra library file format (Ex: "tsv" or "csv")
>python blib2openswath.py --infile data\library\Spectral_library.blib --fasta data\database\sequence.fasta --tol 0.02 --mz_type mono
For more information, post an issue or send an email to chinnu.kemmaai@gmail.com