/RNA-Seq-Analysis

The analytic pipeline used for RNA Sequencing

Primary LanguageRGNU General Public License v2.0GPL-2.0

NOTE

The code here uses the old RNA Seq pipeline. Nowaday, it is better to use tools such as Salmon and tximport as suggested by the big names in RNA Seq. For more details, you can read here

RNA-Seq-Analysis

The programming codes use for RNA Sequencing analysis including the use of STAR, htseq-count, DESeq2 and WGCNA.

perSampleProcess.pl will automatically generate bash scripts for the analysis where user can distribute the jobs manually for maximum efficiency

generateMatrix.pl is used after perSampleProcess.pl so that a count matrix can be generated for down-stream analysis

deAnalysis.r is the Rscript used for the DE analysis. It will try to resolve the dependency wherever possible.

wgcna.r is the Rscript used for the WGCNA analysis. It will try to resolve the dependency wherever possible

Recommended Reference

Reference Fasta ftp://ftp.ensembl.org/pub/release-74/fasta/mus_musculus/dna/Mus_musculus.GRCm38.74.dna.primary_assembly.fa.gz

Reference GTF ftp://ftp.ensembl.org/pub/release-74/gtf/mus_musculus/Mus_musculus.GRCm38.74.gtf.gz

Depending Softwares

STAR https://code.google.com/p/rna-star/

htseq-count http://www-huber.embl.de/users/anders/HTSeq/

R http://cran.r-project.org/