MULTIseqDemux error
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Hi Chris,
I am using the function MULTIseqDemux() within Seurat (v.3.2.2) to demulplex the HTO object.
The command which threw the error:
hto_obj = MULTIseqDemux(hto_obj, assay = "HTO", autoThresh=TRUE)
ERROR:
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
No threshold found for HTO3...
Iteration 1
Using quantile 0.1
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
Iteration 2
Using quantile 0.1
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
No threshold found for HTO1...
No threshold found for HTO2...
No threshold found for HTO3...
Error in if (n_bc_calls[i] == 0) { :
missing value where TRUE/FALSE needed
In addition: Warning messages:
1: In min(x) : no non-missing arguments to min; returning Inf
2: In max(x) : no non-missing arguments to max; returning -Inf
3: In min(x) : no non-missing arguments to min; returning Inf
4: In max(x) : no non-missing arguments to max; returning -Inf
5: In min(x) : no non-missing arguments to min; returning Inf
6: In max(x) : no non-missing arguments to max; returning -Inf
I also attached you the hto_obj file here.