Nanopore analysis of CO-transcriptional processing (nano-COP) exposes the nascent transcriptome via stringent purification of nascent RNAs and direct long-read RNA-sequencing through nanopores. Nano-COP reveals the physical distance between transcription and splicing as well as splicing order and coordination across multi-intron transcripts.
This repository includes the scripts and annotation files needed to analyze nano-COP data. The "annotation_files" folder contains gene feature coordinates from human and Drosophila datasets that are utilized for analyzing nano-COP datasets. The "scripts" folder includes the python code to make necessary datasets and plots for each figure in the nano-COP articles.
For more details on the nano-COP method:
-Our initial publication in Molecular Cell describing the method and its applications: https://www.cell.com/molecular-cell/fulltext/S1097-2765(19)30865-2
-Our step-by-step nano-COP protocol: https://www.nature.com/articles/s41596-020-00469-y