Tool to assists primer design in difficult contexts, e.g. on an alignment of hundreds of sequences and/or on hypervariable genes
The tool uses as input the fasta sequence of an alignment and identifies the best regions for primer design: two low variable regions flanking a highly variable one
Web version of the tool: https://skynet.unimi.it/index.php/tools/easyprimer/
Full user Manual: https://skynet.unimi.it/wp-content/uploads/Maunal_EasyPrimer_online.pdf
If you are using EasyPrimer for an article or scientfic publication please cite:
Perini, M., Piazza, A., Panelli, S. et al. EasyPrimer: user-friendly tool for pan-PCR/HRM primers design. Development of an HRM protocol on wzi gene for fast Klebsiella pneumoniae typing. Sci Rep10, 1307 (2020). https://doi.org/10.1038/s41598-020-57742-z