davidak

davidak.de

Meine persönliche Webseite

minetest

Minetest, an open source infinite-world block sandbox game with survival and crafting. [also https://github.com/minetest/minetest_game]

nixos-config

NixOS configurations for server and desktop systems, including user specific config. with Home Manager

notytdl

Fuck the RIAA, this time with full commit history and all branches.

python-peniscoin-miner

a basic peniscoin miner in python

PyZufall

Generiert zufällige Daten wie Namen, Berufe, Bandnamen, ein Datum, Sätze oder den Datensatz einer Personen

random-vcard-generator

Das Kommandozeilen-Tool generiert VCards mit zufälligen, aber plausiblen Daten.

satzgenerator

Satzgeneratoren in verschiedenen Programmiersprachen

wortliste

Deutsche Wortliste reproduzierbar erzeugen

davidak/PyZufall

Generiert zufällige Daten wie Namen, Berufe, Bandnamen, ein Datum, Sätze oder den Datensatz einer Personen

davidak/notytdl

Fuck the RIAA, this time with full commit history and all branches.

davidak/2023.nixcon.org

NixCon 2023 conference website

davidak/aarch64-build-box

Config for the Community aarch64 NixOS box [maintainer=@grahamc]

davidak/ansible-elasticsearch

Ansible playbook for Elasticsearch

davidak/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273

RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.

davidak/awesome-uses

A list of /uses pages detailing developer setups, gear, software and configs.

davidak/beta-software

AXIOM Beta Software

davidak/ctreffos-webseite

davidak/cvtools

Scripts to get stats and validate data from Mozilla's Common Voice project.

davidak/davidak

davidak/dit

A console text editor for Unix systems that you already know how to use

davidak/effitask

Graphical task manager, based on the todo.txt format.

davidak/fbgrab

fbgrab linux framebuffer screenshot utility.

davidak/fbmark

Linux Framebuffer Benchmark

davidak/infographics

I collect infographics I created here to provide the vector (or whatever source) variant while the png is in the wilds.

davidak/isso

a Disqus alternative

davidak/mobile-nixos

davidak/nix

Nix, the purely functional package manager

davidak/nix.dev

An opinionated guide for developers wanting to get things done with the Nix ecosystem.

davidak/nixos-homepage

Sources for nixos.org

davidak/NixOS-Users-Against-MIC-Sponsorship.github.io

davidak/nixpkgs

Nix Packages collection

davidak/repology-rules

Package normalization ruleset for Repology

davidak/small

Smallest possible syntactically valid files of different types

davidak/tenderowlcom

TenderOwl website

davidak/the-algorithm

Source code for Twitter's Recommendation Algorithm

davidak/trustix

Trustix: Distributed trust and reproducibility tracking for binary caches

davidak/TTS

🐸💬 - a deep learning toolkit for Text-to-Speech, battle-tested in research and production

davidak/woodpecker

Woodpecker is a community fork of the Drone CI system.