Pinned Repositories
akeneo-pim
Spryker Eco Akeneo PIM Module
Aoe_Scheduler
Cron Scheduler Module for Magento
AvS_DisableModules
Adds a shell command info:dependencies:show-removable which exports all modules which have no dependencies
git-tools
simple php git wrapper
magento2
All Submissions you make to Magento Inc. (“Magento") through GitHub are subject to the following terms and conditions: (1) You grant Magento a perpetual, worldwide, non-exclusive, no charge, royalty free, irrevocable license under your applicable copyrights and patents to reproduce, prepare derivative works of, display, publically perform, sublicense and distribute any feedback, ideas, code, or other information (“Submission") you submit through GitHub. (2) Your Submission is an original work of authorship and you are the owner or are legally entitled to grant the license stated above. (3) You agree to the Contributor License Agreement found here: https://github.com/magento/magento2/blob/master/CONTRIBUTOR_LICENSE_AGREEMENT.html
magento2-dynamic-component-registry
Magento2 Module to register Components dynamically in the Backend
magento2-patches
Composer patches for Magento 2
magento2-teaser
Magento2 Module for managing and displaying Teaser Elements
magento2-whoops
rancher-cattle-magento-catalog
davidverholen's Repositories
davidverholen/magento2-dynamic-component-registry
Magento2 Module to register Components dynamically in the Backend
davidverholen/magento2-teaser
Magento2 Module for managing and displaying Teaser Elements
davidverholen/magento2-patches
Composer patches for Magento 2
davidverholen/rancher-cattle-magento-catalog
davidverholen/AvS_DisableModules
Adds a shell command info:dependencies:show-removable which exports all modules which have no dependencies
davidverholen/magento2
All Submissions you make to Magento Inc. (“Magento") through GitHub are subject to the following terms and conditions: (1) You grant Magento a perpetual, worldwide, non-exclusive, no charge, royalty free, irrevocable license under your applicable copyrights and patents to reproduce, prepare derivative works of, display, publically perform, sublicense and distribute any feedback, ideas, code, or other information (“Submission") you submit through GitHub. (2) Your Submission is an original work of authorship and you are the owner or are legally entitled to grant the license stated above. (3) You agree to the Contributor License Agreement found here: https://github.com/magento/magento2/blob/master/CONTRIBUTOR_LICENSE_AGREEMENT.html
davidverholen/magento2-whoops
davidverholen/akeneo-pim
Spryker Eco Akeneo PIM Module
davidverholen/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
davidverholen/b2b-demo-shop
Spryker B2B Demo Shop based on the Spryker Commerce OS
davidverholen/catalog
[READ ONLY] Subtree split of the Catalog module.
davidverholen/CleanCheckout
A drop-in replacement for the Magento 2 checkout.
davidverholen/CopeX_VatFix
Magento2 VAT Check with valid countrycodes in UID as usual in European Union | pendant to Multibyte_VatFix for Magento1
davidverholen/firegento-magesetup2
MageSetup for Magento2
davidverholen/graphql-ce
Magento 2 GraphQL community project
davidverholen/k8sdemo
davidverholen/mage2-module-boilerplate
davidverholen/magento-2
PAYONE Payment Extension for Magento 2
davidverholen/magento2-blog
davidverholen/magento2-cms
davidverholen/magento2-example-uicomponent-mixin
Example Magento 2 module creating a RequireJS mixin for the UiComponent class
davidverholen/magento2-fix13929
A fix for 13929 while we wait for core to patch/release stuff.
davidverholen/magento2-mage-god
A stupid but educational example to show that the Mage god-class can be used in M2 too
davidverholen/magento2-module
FACT-Finder® Web Components for Magento 2
davidverholen/magento2-theme-blank-sass
SASS based version of Magento 2 Blank theme
davidverholen/Magento2_German_LocalePack_de_DE
Deutsches Sprachpaket für Magento 2 Community Edition
davidverholen/mc-magento2
MailChimp for Magento 2
davidverholen/pwa-studio
🛠Development tools to build, optimize and deploy Progressive Web Applications for Magento 2.
davidverholen/rancher-catalog
davidverholen/vue-storefront-api
Vue.js storefront for Magento2 (and not only) - data backend