No valid read to estimate the length distribution!

[TdzBAS]

I get following error message, even after post-alignment quality control with STAR when i use rsem-calculate-expression:

my commands are :

rsem-calculate-expression --bam --no-bam-output -p 8 --paired-end ${filename} Simulation/ref/reference Simulation/data

get the same error when i use the paired fasta files directly.

anyone with an idea?

[TdzBAS]

UPDATE:
my original input were fasta files and not fastq-files, therefore adding the flag --no-qualities resolved the error and the issue can be closed!