digitalcytometry/cytotrace2

Unable to open: No such file or directory (2) Unable to open: No such file or directory (2) Error in if (fsize > 2147483647) { : 需要TRUE/FALSE值的地方不可以用缺少值

Baozi9 opened this issue · 2 comments

Baozi9 commented

Hi， When I just run the plot function , it come with the following problem?
Can anyone help me to deal it? thanks a lot！！！！！！！！！

plots <- plotData(cytotrace2_result = cytotrace2_result,

  •               annotation = annotation, 

  •               is_seurat = TRUE)

Preparing input for visualization.
Performing log-normalization
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variances
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
|=========================================================| 100%
Unable to open: No such file or directory (2)
Unable to open: No such file or directory (2)
Error in if (fsize > 2147483647) { : 需要TRUE/FALSE值的地方不可以用缺少值

savagyan00 commented

Hi,

Thank you for using CytoTRACE 2 and reaching out with this issue.

To better assist you, could you please confirm a few details about the steps leading up to this error?

  • How was the cytotrace2() function called? Please provide the exact command used, if possible.
  • If cytotrace2_result is a Seurat object, could you please confirm that the expression data is accessible from the RNA assay and that your annotation input is a data.frame object?

If possible, sharing the input data that generates this error could help us diagnose the issue more effectively. You can send the details to cytotrace2team@gmail.com.

We appreciate your cooperation!

savagyan00 commented

Hello,

We're closing this issue due to inactivity, but please feel free to reach out if you continue to experience the problem or have any other questions.

We're here to help!