epi2me-labs/modbam2bed

Working with Dorado Basecaller

Closed this issue · 1 comments

Does this tool only work with the Guppy basecaller? I used the Dorado basecaller like so:

@PG     ID:basecaller   PN:dorado       VN:0.1.1+eb48766        CL:dorado basecaller dorado-0.1.1/dna_r10.4.1_e8.2_400bps_hac@v4.0.0 fast5s --modified-bases 5mCG_5hmCG

I can't seem to get it to work, although the test data works just fine. Thanks for the help in advance!

cjw85 commented

The program will work with any BAM file containing aligned reads and MM and ML tags.