/SCVI_Heart

Singel Cell Variational Analysis (SCVI) of the heart cell atlas data. reproduction of nature paper

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❤️🔬 SCVI_Heart

or single cell variational inference of the Human Heart. here I reproduce a single-cell variational model and analysis of the Nature paper of Litviňuková et al. (2020).

📄 Paper:

Litviňuková, M., Talavera-López, C., Maatz, H., Reichart, D.,Worth, C. L., Lindberg, E. L., … & Teichmann, S. A. (2020). Cells of the adult human heart. Nature, 588(7838), 466-472.

📊 Data:

single cells, nuclei and CD45+ enriched cells from the left and right ventricular free walls, left and right atrium, the left ventricular apex, and interventricular septum, from 14 adults. After processing with 10X Genomics and a generative deep variational autoencoder, the resulting dataset comprises 45,870 cells, 78,023 CD45+ enriched cells and 363,213 nuclei for 11 major cell types:"

  • atrial cardiomyocytes,
  • ventricular cardiomyocytes,
  • fibroblasts (FBs),
  • endothelial cells (ECs),
  • pericytes, smooth muscle cells (SMCs),
  • immune cells (myeloid and lymphoid),
  • adipocytes,
  • mesothelial cells
  • and neuronal cells

🎯 Goal:

mapping out the genomics data as represemted by Litviňuková et al. (2020) in Nature.