DeBruijnBloom: An HTML repository from fsaina

Faculty of Electrical Engineering and Computing Bioinformatics course projects (https://www.fer.unizg.hr/en/course/bio)

Replication of paper: Space-efficient and exact de Bruijn graph representation based on a Bloom filter ( https://almob.biomedcentral.com/articles/10.1186/1748-7188-8-22 )

Setup project

Run sh ./INSTALL.sh to setup the project. The script will:

Create ./bin directory
Download the Jellyfish executable (version 2.2.10 for Linux) into ./bin directory
Compile the project with g++ -std=c++14 main.cpp BloomFilter.cpp ExactDeBruijnGraph.cpp KmerUtil.cpp MurmurHash3.cpp measures.cpp -o ./bin/DeBrujinBloom

Run project

Execution example: ./bin/DeBrujinBloom -k 21 --minAbundance 3 --maxBreadth 20 --maxDepth 500 --input data/ecoli/ecoli.fasta For more information run ./bin/DeBrujinBloom --help

Run test example

To run test example:

Run g++ -std=c++14 Demo.cpp -o bin/Demo to compile
Run ./bin/Demo to execute

Data

The E.Coli genome (data/ecoli.fasta) was taken from http://bacteria.ensembl.org/Escherichia_coli_14a/Info/Index/

To create custom synthetic genome sequences:

Make sure ./data/generate.sh is executable, otherwise run chmod +x ./data/generate.sh
Run ./data/generate.sh N > ./data/<NAME_OF_FILE>.fasta (e.g. ./data/generate.sh 100 > ./data/uniform_100.fasta)

To create the synthetic genome sequences(E.Coli):

Compile wgsim tool gcc -g -O2 -Wall -o ./bin/wgsim ./wgsim/wgsim.c -lz -lm
For creating synthetic sequences of length x, with error y, run ./bin/wgsim -1 x -d0 -S11 -e0 -r y ./data/Escherichia_coli.fa ./data/read-x-y.fq /dev/null

Testing

For testing purposes we used 'blastn' and 'valgrind' tools, and made our implementation of N50 measure(see in next section).

To use blastn(used to check matching of generated sequences and original data):

Download and compile blastn and put it in bin/ directory
Generate some outputs in fasta format
Run ./bin/blastn -query path-to-generated-data.fasta -subject path-to-original-sequence.fasta -out results.txt

To use Valgrind tool(used to get a memory usage report):

Download and install Valgrind
Run valgrind ./bin/DeBrujinBloom program_arguments

We downloaded the original implementation of this paper at http://minia.genouest.org/, so we can test their performances:

Download and compile minia, move it to /bin directory
Run ./bin/minia path-to-fasta-file k min-abundance genome-length output-file-name

Measure

To calculate the N50 measure from the execution output file, either:

Add a "-n 1" flag when calling ./bin/DeBrujinBloom
Or run the N50.py python script with grep "^>" ./output/output.fasta | cut -d "_" -f5 | ./N50.py (Don't forget to make N50.py executable with chmod +x)