/NGS-scripts

Scripts for next generation sequencing analyses

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NGS-scripts

Repository for scripts used for analysing next generation sequencing data organised by technique.

At_rRNA_AGIs.txt

List of rRNA loci for removal prior to DEG calling.

TAIR10_annotation.sh

Download TAIR10 gff and produce annotation files

TruSeq-adapters.fa

FASTA file containing illumina adapter sequences for scythe step in RNA-seq alignment.

Araport11_assemble.sh

Build annotation files based on araport11 gff.

average_cov.sh

Calculate average depth using samtools depth on sorted BAM file.

gene_to_gene_anno.sh

Set of commands (bash & R) to take TAIR GFF files and produce annotation files, here specifically making a annotation file of genes in tandem orientation.