galaxyproject/usegalaxy-tools

Repair Classify.seqs at ORG

Closed this issue · 1 comments

tool_id=toolshed.g2.bx.psu.edu/repos/iuc/mothur_classify_seqs/mothur_classify_seqs/1.39.5.0

Test history https://usegalaxy.org/u/jen-galaxyproject/h/imported-from-archive-training-16s-rrna-sequencing-with-mothur-main-tutorial-imported-from-url----classifyseqs-purges-tax-input

Test workflow -- same as GTN Tutorial https://training.galaxyproject.org/training-material/topics/microbiome/tutorials/mothur-miseq-sop/workflows/

What was done:

  1. Load up tutorial inputs
  2. Run the workflow at EU
  3. Export History from EU > Import to ORG (history), Download WF from EU > Upload to ORG
  4. Run the workflow at ORG
  5. See the bug .. the tax input is purged.

This usage makes use of the converter tool CONVERTER_ref_to_seq_taxomony but when I run that directly in a different test history, it works fine. https://usegalaxy.org/u/jen-galaxyproject/h/test-ref-to-seq

I moved the WF from EU > ORG but this also happens if the workflow is directly loaded from GTN. This was a cross test to see if the WF was the problem or not -- seems not, works at EU but not at ORG.

Same happened in Dec 2023 but it seemed like a fluke, see https://usegalaxy.org/u/jen-galaxyproject/h/training-16s-rrna-sequencing-with-mothur-main-tutorial-3

Did work Ok in Nov 2023 https://usegalaxy.org/u/jen-galaxyproject/h/training-16s-rrna-sequencing-with-mothur-main-tutorial-2

So something happened between Nov 27 2023 and Dec 13 2023 to impact this tool. No successful runs since then.

Started up another just to see what happens https://usegalaxy.org/u/jen-galaxyproject/h/training-16s-rrna-sequencing-with-mothur-main-tutorial-imported-from-url-imported-from-uploaded-file

The rerun here worked Ok. Not sure what changed but isn't reproducible now.

Started up another just to see what happens https://usegalaxy.org/u/jen-galaxyproject/h/training-16s-rrna-sequencing-with-mothur-main-tutorial-imported-from-url-imported-from-uploaded-file

Any end-users following should also try a re-run now.