David Aciole Barbosa About the gff and fasta files; >>>>Gff gff file "Pb18-GFF-2019+NC-sorteddedup_final.gff" was parsed with the custom script "deduplicate-GFF-IDs-v2.sh" in order to rename some features like CDS wich contained duplicated IDs. The original gff was downloaded from ParaDB at http://paracoccidioides.com/paracoccidioides-brasiliensis-pb18-downloads/. Machado does not support dupplicated features IDs because they are used as primary keys. >>>>Fasta was downloaded from OSF repository "Transcriptomic profiling identifies novel transcripts, isomorphs and lncRNAs in Paracoccidioides brasiliensis" at https://osf.io/yn4ra/. >>>> Latest NCBI GFF GCF_000150735.1_Paracocci_br_Pb18_V2_genomic.gff.gz https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/150/735/GCF_000150735.1_Paracocci_br_Pb18_V2/GCF_000150735.1_Paracocci_br_Pb18_V2_genomic.gff.gz Downloaded April 11, 2021 >>>> Latest Ensembl Fungi GFF Paracoccidioides_brasiliensis_pb18_gca_000150735.Paracocci_br_Pb18_V2.50.gff3.gz ftp://ftp.ensemblgenomes.org/pub/fungi/release-50/gff3/fungi_ascomycota1_collection/paracoccidioides_brasiliensis_pb18_gca_000150735/Paracoccidioides_brasiliensis_pb18_gca_000150735.Paracocci_br_Pb18_V2.50.gff3.gz Downloaded April 11, 2021 >>>> Latest Ensembl Fungi genome fasta Paracoccidioides_brasiliensis_pb18_gca_000150735.Paracocci_br_Pb18_V2.dna.nonchromosomal.fa.gz ftp://ftp.ensemblgenomes.org/pub/fungi/release-50/fasta/fungi_ascomycota1_collection/paracoccidioides_brasiliensis_pb18_gca_000150735/dna/Paracoccidioides_brasiliensis_pb18_gca_000150735.Paracocci_br_Pb18_V2.dna.nonchromosomal.fa.gz Downloaded April 12, 2021