Pinned Repositories
A-genetic-network-for-host-control-of-phyllosphere-microbiota-for-plant-health
The phyllosphere (above-ground parts) of plants are essential for photosynthesis, crop productivity and ecosystem health, and represents one of the most abundant habitats for microbiota colonization on earth. Yet, how plants control phyllosphere microbiota for health and productivity remains poorly understood at the mechanistic level. Here we found that the Arabidopsis quadruple mutant (min7 fls2 erf cerk1 [mfec]) defective in pattern-triggered immunity and the MIN7 vesicle traffic pathway or the constitutively activated cell death1 (cad1) mutant defective in a membrane attack complex/perforin (MACPF) domain protein displayed poor phyllosphere health. The relative abundance of Firmicutes was dramatically reduced, whereas Proteobacteria are enriched in the mfec and cad1 mutants, bearing a striking cross-kingdom resemblance to what occurs in human inflammatory bowel disease. Synthetic community reconstruction and community transplantation experiments revealed a causal role of a diverse and healthy level of leaf bacterial community in phyllosphere health. Our results uncovered an evolutionarily ancient molecular framework that allows terrestrial plants to control a diverse, balanced endophytic leaf microbiota to ensure phyllosphere health.
Mycorrhizal-symbiosis-modulates-the-rhizosphere-microbiota-to-promote-rhizobia-legume-symbiosis
Plants establish symbioses with mutualistic fungi, such as arbuscular mycorrhizal (AM) fungi, and bacteria, such as rhizobia, to exchange key nutrients and thrive. The plants and symbionts have coevolved and represent vital components of terrestrial ecosystems. Plants employ an ancestral AM signaling pathway to establish intracellular symbioses, including the legume-rhizobia symbiosis, in their roots. Nevertheless, the relationship between the AM and rhizobial symbioses in native soil is poorly understood. Here, we examined how these distinct symbioses affect root-associated bacterial communities in Medicago truncatula, by quantitative microbiota profiling (QMP) of 16S rRNA genes. We found that M. truncatula mutants that cannot establish AM or rhizobia symbiosis have an altered microbial load (quantitative abundance) in rhizosphere and roots, and in particular that AM symbiosis is required to assemble a normal quantitative root-associated microbiota in native soil. Moreover, quantitative microbial co-abundance network analyses revealed that the AM symbiosis impacts Rhizobiales-hubs among the plant microbiota and benefit the plant holobiont. Through QMP of rhizobial rpoB and AM fungal SSU rRNA genes, we revealed a new layer of interaction, whereby AM symbiosis promotes rhizobia accumulation in the rhizosphere of M. truncatula. We further showed that AM symbiosis-conditioned microbial communities within the M. truncatula rhizosphere could promote nodulation in different legume plants in native soil. Given that the AM and rhizobial symbioses are critical for crop growth, our findings might inform strategies to improve agricultural management. Moreover, our work sheds light on the co-evolution of these intracellular symbioses during plant adaptation to native soil conditions.
Whole-plant-microbiome-profiling-reveals-a-novel-geminivirus-associated-with-soybean-stay-green-dise
The R scripts employed for "Whole plant microbiome profiling reveals a novel geminivirus associated with soybean stay-green disease"
godlovexiaolin's Repositories
godlovexiaolin/Whole-plant-microbiome-profiling-reveals-a-novel-geminivirus-associated-with-soybean-stay-green-dise
The R scripts employed for "Whole plant microbiome profiling reveals a novel geminivirus associated with soybean stay-green disease"
godlovexiaolin/Mycorrhizal-symbiosis-modulates-the-rhizosphere-microbiota-to-promote-rhizobia-legume-symbiosis
Plants establish symbioses with mutualistic fungi, such as arbuscular mycorrhizal (AM) fungi, and bacteria, such as rhizobia, to exchange key nutrients and thrive. The plants and symbionts have coevolved and represent vital components of terrestrial ecosystems. Plants employ an ancestral AM signaling pathway to establish intracellular symbioses, including the legume-rhizobia symbiosis, in their roots. Nevertheless, the relationship between the AM and rhizobial symbioses in native soil is poorly understood. Here, we examined how these distinct symbioses affect root-associated bacterial communities in Medicago truncatula, by quantitative microbiota profiling (QMP) of 16S rRNA genes. We found that M. truncatula mutants that cannot establish AM or rhizobia symbiosis have an altered microbial load (quantitative abundance) in rhizosphere and roots, and in particular that AM symbiosis is required to assemble a normal quantitative root-associated microbiota in native soil. Moreover, quantitative microbial co-abundance network analyses revealed that the AM symbiosis impacts Rhizobiales-hubs among the plant microbiota and benefit the plant holobiont. Through QMP of rhizobial rpoB and AM fungal SSU rRNA genes, we revealed a new layer of interaction, whereby AM symbiosis promotes rhizobia accumulation in the rhizosphere of M. truncatula. We further showed that AM symbiosis-conditioned microbial communities within the M. truncatula rhizosphere could promote nodulation in different legume plants in native soil. Given that the AM and rhizobial symbioses are critical for crop growth, our findings might inform strategies to improve agricultural management. Moreover, our work sheds light on the co-evolution of these intracellular symbioses during plant adaptation to native soil conditions.
godlovexiaolin/A-genetic-network-for-host-control-of-phyllosphere-microbiota-for-plant-health
The phyllosphere (above-ground parts) of plants are essential for photosynthesis, crop productivity and ecosystem health, and represents one of the most abundant habitats for microbiota colonization on earth. Yet, how plants control phyllosphere microbiota for health and productivity remains poorly understood at the mechanistic level. Here we found that the Arabidopsis quadruple mutant (min7 fls2 erf cerk1 [mfec]) defective in pattern-triggered immunity and the MIN7 vesicle traffic pathway or the constitutively activated cell death1 (cad1) mutant defective in a membrane attack complex/perforin (MACPF) domain protein displayed poor phyllosphere health. The relative abundance of Firmicutes was dramatically reduced, whereas Proteobacteria are enriched in the mfec and cad1 mutants, bearing a striking cross-kingdom resemblance to what occurs in human inflammatory bowel disease. Synthetic community reconstruction and community transplantation experiments revealed a causal role of a diverse and healthy level of leaf bacterial community in phyllosphere health. Our results uncovered an evolutionarily ancient molecular framework that allows terrestrial plants to control a diverse, balanced endophytic leaf microbiota to ensure phyllosphere health.