invalid feature coordinates from long read 'collapsed' assembly

[noncodo]

So I 'collapsed' some long read data into a gtf with :
/home/apps/stringtie2.1.4/stringtie ${BAMLIST} -p ${THREADS} -L -R -o hg38_merged.gtf
I then try to quantify each sample/bam against this cleaned and collapsed reference, but get several

Error: invalid feature coordinates (end<start!) at line:
chr1 StringTie exon 21220887 21220732 1000 - . gene_id "STRG.8348"; transcript_id "STRG.8348.1"; exon_number "1";

type messages.
What's up with that?
My bams are from minimap2.

[xue-222]

Did you solve the problem?

[noncodo]

We manually remove the erroneous entries. Not an ideal solution.

…

On Apr 24, 2023, at 8:51 AM, xue-222 ***@***.***> wrote: Did you solve the problem? — Reply to this email directly, view it on GitHub <#338 (comment)>, or unsubscribe <https://github.com/notifications/unsubscribe-auth/ABDCR3ZAKWEPEDTYFF3TY6TXCZZMJANCNFSM5CPEEMAQ>. You are receiving this because you authored the thread.

[xue-222]

Error: invalid feature coordinates (end<start!) at line:
chr12 transcriptome mRNA 1197998703 1.198e+09 . + . ID=PtQG36900.3;Parent=PtQG36900
I made such an error, and I tried to convert scientific notation.