Nextflow test workflow

A Nextflow workflow that runs FastQC on Fastq reads and then combines them into a MultiQC report. FastQC and MutliQC are setup on a Docker container. The nextflow script pulls the Docker container and converts it to a Singularity container on the fly. See the nextflow.log for settings to run on a Slurm cluster.

To be able to mount the /data directory on the cluster you need to have the mount defined in your Docker/Singularity container e.g. see

To run

In the nextflow.config two possible ways to pull the container are suggested. Directly from quay.io or directly from a file on the share.

To be able to mount the non native singularity conainer mounts e.g. data singularity.autoMounts = true should be set.

nextflow -log nextflow.log run -w /data/users/cbio-ws-u24/nextflow-workdir -c nextflow.config main.nf -profile slurm

$ squeue 
             JOBID PARTITION     NAME     USER ST       TIME  NODES NODELIST(REASON)
              2324      main tl.rFQC. cbio-ws-  R       0:12      1 cbio-ws-1
              2325      main tl.rFQC. cbio-ws-  R       0:12      1 cbio-ws-1
              2327      main tl.rFQC. cbio-ws-  R       0:12      1 cbio-ws-1
              2329      main tl.rFQC. cbio-ws-  R       0:12      1 cbio-ws-1
              2336      main tl.rFQC. cbio-ws-  R       0:12      1 cbio-ws-1

Accessing the container and running a command

Get onto a node

srun -N 1 --ntasks-per-node=1 --pty bash

Get int the container (specify the /data bind)

singularity exec -B /data:/data /data/projects/cbio-ws/tests/containers/quay.io_h3abionet_org_h3a16s-fastqc.img bash

Run command in container

fastqc /data/projects/cbio-ws/tests/fastq-reads/Dog1_R1.fastq -o /data/users/cbio-ws-u24/

Check if data is generated on the cluster (exit container and do listing)

exit 
ls /data/users/cbio-ws-u24/Dog1_R1_fastqc.*
/data/users/cbio-ws-u24/Dog1_R1_fastqc.html  /data/users/cbio-ws-u24/Dog1_R1_fastqc.zip

grbot/run-fastqc