hansenjn/CiliaQ

CiliaQ produces empty output file

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CiliaQ constantly produces empty output txt and no skeleton file for certain file (provided privately).

This issue was not originating from CiliaQ but from the configuration of the file. The file was supposed to be handled as a "3D stack" with multiple slices and one timepoint but was erroneously defined as a "timelapse 2D stack" with one slice and multiple timepoints. In ImageJ, this is visualized at the bars below the image:

  • This is a stack with multiple channels and multiple time steps (see play button):
    grafik
  • This is a stack with multiple channels and mulitple slices (see "z"):
    grafik

As a note: For timelapse stacks, CiliaQ only outputs time-averages for a cilium in the main output file, if the cilium is present in all time frames.