he-hai/enzymatic-tools

This repository contains scripts for enzyme kinetic parameters calculation.

kinecalc.py is for Michaelis–Menten kinetics $v=\frac{Vmax*[S]}{Km+[S]}$.

And kinecalc_subI.py is for kinetics with substrate inhibition $v=\frac{Vmax*[S]}{Km+[S]+\frac{[S]^2}{Ki}}$.

The scripts use lmfit for non-linear curve fitting, pint for units handling.

The scripts have been tested in an environment with following packages:

• python 3.8.2
• numpy 1.23.4
• pandas 1.3.4
• matplotlib 3.5.1
• seaborn 0.11.2
• lmfit 1.0.0
• openpyxl 3.0.6
• scipy 1.4.1
• pint 0.17