horsetery2017
New media artist, Write poetry and code, Pay attention to the mapping of objects in imagination, Also pay attention to the construction of objects in reali.
Pinned Repositories
10kinds-light-face-detector-align-recognition
10种轻量级人脸检测算法的比拼,其中还包含人脸关键点检测与对齐,人脸特征向量提取和计算距离相似度
3D-Machine-Learning
A resource repository for 3D machine learning
ARKIT2MAYA
arkit2maya
bookget
bookget 数字古籍图书下载工具
FaceCap
Realtime motion capture server for Autodesk Maya, developed in Xantus Animation Studio.
grab_frame
A blender addon for fast stepping through multiple frames.
maya-script
animation toool
python-shape-grammars
Shape Grammar Programming writtien in Python
shape_grammar_compiler
As a principle verification of the shape grammar
horsetery2017's Repositories
horsetery2017/LaTeX-examples
Examples for the usage of LaTeX
horsetery2017/3D-Machine-Learning
A resource repository for 3D machine learning
horsetery2017/Blender-Addon-Photogrammetry-Importer
Addon to import different photogrammetry formats into Blender
horsetery2017/books-1
【编程随想】收藏的电子书清单(多个学科,含下载链接)
horsetery2017/animextras
An open source Blender addon that adds extra animation functionality.
horsetery2017/python-shape-grammars
Shape Grammar Programming writtien in Python
horsetery2017/rpi_pose_estimation
Code to run pose estimation on the Raspberry Pi 4 using TensorFlow Lite and Postnet!
horsetery2017/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
horsetery2017/DALL-E
PyTorch package for the discrete VAE used for DALL·E.
horsetery2017/monster-mash
horsetery2017/ShenzenAirport
3D Model of the Shenzen Aiport - developed in Racket using the Rosetta tool in DrRacket, generated and rendered in AutoCAD, and structural analysis performed in Robot Structural Analysis..
horsetery2017/PyMesh
Geometry Processing Library for Python
horsetery2017/wfc_2019f
horsetery2017/WaveFunctionCollapse
Bitmap & tilemap generation from a single example with the help of ideas from quantum mechanics
horsetery2017/wfc_2D_B3D
2D Wave Function Collapse as Blender Plugin
horsetery2017/gov-takedowns
Text of government takedown notices as received. GitHub does not endorse or adopt any assertion contained in the following notices.
horsetery2017/Godot_ARMory
A script to help build Godot Game Engine 4.0 on ARM Linux machines.
horsetery2017/tinyrenderer
A brief computer graphics / rendering course
horsetery2017/opentoonz
OpenToonz - アニメーション制作ツールのオープンソースプロジェクト
horsetery2017/R8-cs1.6
very very old source code for cs 1.6, from CSX Community
horsetery2017/ofxGuiExtended
ofParameter based GUI addon for openFrameworks; derived from the core OF ofxGui addon and ofxDOM.
horsetery2017/Quake-III-Arena
Quake III Arena GPL Source Release
horsetery2017/Scotty3D
3D graphics software for mesh editing, path tracing, and animation
horsetery2017/ProceduralMeshDemos
Examples of procedural mesh generation in Unreal 4.
horsetery2017/norman
A WebVR animation tool by James Paterson.
horsetery2017/graphics-driver-samples
This repository contains graphics driver samples used to demonstrate how to write graphics driver for the windows platform.
horsetery2017/animation_nodes
Node based visual scripting system designed for motion graphics in Blender.
horsetery2017/khadas.github.io
Colorful Khadas
horsetery2017/3DWorld
3D Procedural Game Engine Using OpenGL
horsetery2017/blender
Official mirror of Blender