/Acclim_Dynamics

Holobiont Integration Acclimatization Dynamics

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Coral species loss and physiological legacy effects are elicited by extended marine heatwave and projected ocean acidification conditions

Emma Strand, Kevin Wong, Alexa Farraj, Sierra Gray, Ana McMenamin, and Hollie Putnam. [Putnam Lab, University of Rhode Island].

This repository contains data, scripts, protocols, and analyses for physiological responses and Symbiodiniaceae community compositions of Montipora capitata and Pocillopora acuta during a simulated extended heatwave and ocean acidification stress and recovery time-series. This work was conducted in 2018 at the Hawaii Institute of Marine Biology.

Figure 1

Figure1

Data Organization

This repository is organized by Output, Protocols, Scripts, data, and metadata.

data is categorized into subfolders: Environmental, ITS2_seq, and Physiological_variables. Environmental includes all temperature, pH, light, flow rate data, and total alkalinity measurements of experimental tanks.

Physiological_variables folder include data for the following:

  • Survivorship
  • Color Score
  • Growth Rates (via Buoyant Weight)
  • Respiration rates
  • Photosynthetic rates and photosynthetic irradiance curves
  • Host soluble protein
  • Host total antioxidant capacity
  • Endosymbiont densities
  • Chlorophyll content
  • Host and symbiont tissue biomass

ITS2_seq folder includes all data for Symbiodiniaceae communities analyzed by ITS2 Sequencing. All raw sequences are uploaded to NCBI under PRJNA761780.

Output includes all physiological and Symbiodiniaceae community analyses conducted with R markdown scripts organized in Scripts and figures produced.

Contact

Any questions about this project and the contents of this repository can be directed to Emma Strand at emma_strand (at) uri.edu.