To examine the uptake of Carbon NanoTube (CNT) lipid sensors in coral colonies and cells
To compare the CNT lipid sensor to traditional lipid quantification methods
To quantify lipids in live corals under thermal stress
Produce reliable data on the uptake and longevity of Carbon NanoTube (CNT) lipid sensors in Astrangia colonies and cells
Develop strategy to mitigate autofluorescence under microscope
Examine intracellular lipid content in Astrangia through tissue extractions
Compare the uptake and longevity of Carbon NanoTube (CNT) lipid sensors in different species of coral colonies and cells
Quantify lipids in live corals under thermal stress, and base a proposal on this
Compare the CNT lipid sensor to traditional lipid quantification methods
Test for possible toxicity of CNTs
Test lipid content of bailing out polyp micropropagules through settlement (Pocillopora is the most reliable for now) (if possible)
Test Astrangia larvae through settlement (if possible)
M. Gravely
A. Thorson
H. Putnam
D. Roxbury
H. Reich
Astrangia poculata, Pocillopora acuta, Montipora capitata