Welcome to Moolti, Multiple Organism Optimization Lab Tool for iGEM ! MOOLTi allows for the codon optimization of a DNA sequence for multiple organisms, simultaneously. To learn more , go to http://2018.igem.org/Team:HebrewU/Software MOOLTi is now available online ! optimize your proteins at http://ec2-52-23-211-181.compute-1.amazonaws.com Example .fasta protein files and restriction enzymes files can be found at CodonOptimization\example_files Example .csv files for many model organisms can be found at CodonOptimization\organism_files Usage instructions: Run using Python GUI: 1) Double - Click optimize.bat 2)Pick optimization method 3) upload: a) a .fasta file containing the protein b) an empty file to save the result into c) (optional) a file containing restriction enzymes names, or simply type them in (separated by comma ) 4) click "Next" 5) upload a .csv file containing a codon usage table (see database) for each organism 6) (optional) add up to 4 organisms 7) click "optimize" 8) the file with the output DNA was saved to the desired destination. Command Line Run: 1) open command line 2) Go to the downloaded folder location -> CodonOptimization\ (make sure Main.py is in the same folder !) 3) run (in cmd line): python3 Main.py <protein sequence string> <threshold (defult is 0.05)> <organism 1 codon filename (csv file)> <organism 2 codon table filename (csv file)> 4) press enter 5) write the case sensitive restriction enzymes to be avoided, if none press enter 6) the output is printed to the cmd line