Pinned Repositories
AndroidAnalysis
Simple Bash script for anlaysing APK files
AutOSINT
Tool to automate common OSINT tasks
caldera
Automated Adversary Emulation Platform
client-python
OpenCTI Python client
connectors
OpenCTI connectors
deepdarkCTI
Collection of Cyber Threat Intelligence sources from the deep and dark web
opencti
Open Cyber Threat Intelligence Platform
OSINT_sources
OTHF
Open Threat Hunting Framework
ransomwatch
a ransomware-group observatory 🧅👹
imuledx's Repositories
imuledx/OSINT_sources
imuledx/caldera
Automated Adversary Emulation Platform
imuledx/deepdarkCTI
Collection of Cyber Threat Intelligence sources from the deep and dark web
imuledx/uac
UAC is a Live Response collection script for Incident Response that makes use of native binaries and tools to automate the collection of AIX, Android, ESXi, FreeBSD, Linux, macOS, NetBSD, NetScaler, OpenBSD and Solaris systems artifacts.
imuledx/client-python
OpenCTI Python client
imuledx/connectors
OpenCTI connectors
imuledx/opencti
Open Cyber Threat Intelligence Platform
imuledx/OTHF
Open Threat Hunting Framework
imuledx/ransomwatch
a ransomware-group observatory 🧅👹
imuledx/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
imuledx/AutoGPT
AutoGPT is the vision of accessible AI for everyone, to use and to build on. Our mission is to provide the tools, so that you can focus on what matters.
imuledx/CAPEv2
Malware Configuration And Payload Extraction
imuledx/datasets
OpenCTI datasets
imuledx/detection-rules
Rules for Elastic Security's detection engine
imuledx/elasticsearch_loader
A tool for batch loading data files (json, parquet, csv, tsv) into ElasticSearch
imuledx/ent_random_sequence_tester_ransomware
Utility for testing random and pseudorandom sequences, either as bytes or bit streams, reporting entropy, mean value, serial correlation, chi square, and Monte Carlo estimate of an value, serial correlation, chi square, and Monte Carlo estimate of π.
imuledx/falconpy
The CrowdStrike Falcon SDK for Python
imuledx/falcorules
Falco rule repository
imuledx/IntelOwl
Intel Owl: analyze files, domains, IPs in multiple ways from a single API at scale
imuledx/IntelOwl-ng
Web client for Intel Owl. Built with Angular 9.
imuledx/linux-voice-control
Your personal, fully customization, Linux Voice Control Assistant.
imuledx/malware-bazaar
Python scripts for Malware Bazaar
imuledx/n8n
Free and source-available fair-code licensed workflow automation tool. Easily automate tasks across different services.
imuledx/pyintelowl
Simple Client for Intel Owl
imuledx/rss2slack
Feed of computer security websites and code to push updates to a slack webhook
imuledx/rules
Repository of yara rules
imuledx/SeaMonk
A Smart-Contract gateway for Cardano. Allow users to interact with a smart contract via simple transactions.
imuledx/ThreatIntelligenceDiscordBot
Gets updates from various clearnet domains and ransomware threat actor domains
imuledx/tradingview-interactive-brokers
TradingView Interactive Brokers Integration using Webhooks
imuledx/twint
An advanced Twitter scraping & OSINT tool written in Python that doesn't use Twitter's API, allowing you to scrape a user's followers, following, Tweets and more while evading most API limitations.