/Robust-HSE-Examples

A robust methodology for assessing homoeolog-specific expression

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Robust-HSE-Examples

A robust methodology for assessing homoeolog-specific expression

This code was developed for educational purposes. To execute the pipeline, install the required software and run 'bash robust_HSE_examples.bash'

Example usage for LAST_COREs.py

'''bash usage: LAST_COREs.py [-h] -a LAST_A -b LAST_B

This script is designed to identify reciprocal best-hits given two parsed LAST files. Output includes reciprocated_last_hits.txt and two BED files representing HSP coordinates from LAST_A for query and subject.

    Usage: python LAST_COREs.py -a A_to_B_LAST -b B_to_A_LAST

optional arguments: -h, --help show this help message and exit -a LAST_A, --LAST_A LAST_A Parsed LAST file from species A in tab-separated format. -b LAST_B, --LAST_B LAST_B Parsed LAST file from species B in tab-separated format. '''